In KuNG FU home page, user can access data contained in the database, selecting ‘search’ option that supports free-text searches and ‘browse’ option that allows query type from a predefined scroll-down menu. Section ‘statistics’ provides a graphical representation of the database content and section ‘download/upload’ allows user to export data stored in the database and to upload their own experimentally validated KGF data through a web based submission form.

The query can be typed as a free-text searches in the ‘Filter by gene’ box and immediately provides a summary table with links to the ‘Details’ sections for each Kinase Gene Fusion (KGF) found in the database. By clicking on the ‘Details’ tab, for each KGF an output page organized in ‘Cell line’, ‘Fusion’, ‘AGFusion plot’, ‘Kinase’ and ‘Supporting literature’ sub-sections is displayed. These sub-sections provide detailed KGF molecular information, such as breakpoints and respective specific transcripts and introns/exons involved in the fusion event, along with the AGFusion plot. Each KGF annotation dataset also includes information on published experimental methods used for KGF validation with several supporting literature references and links to the Tumor Fusion Gene Data Portal database for corresponding KGF events detected in patient-derived tumor samples.

Users can interrogate KuNG FU from a predefined scroll-down menu searching for ‘kinase gene name’, ‘kinase group name’, ‘cell line name’, ‘primary tissue’ or by ‘chromosomal rearrangement’ event generating the KGF. Upon filtering, a summary table with links to the ‘Details’ sections is provided for the selected KGFs, reporting cell line, tissue and molecular details such as fusion partner and chromosomal rearrangement event. By clicking on the ‘Details’ tab, an output page contains all the data types collected for each KGF, organized in sub-sections, such as ‘Cell Line’, ‘AGFusion Plot’, ‘Fusion’, ‘Kinase’ and ‘Supporting Literature’ is provided. These sub-sections show detailed KGF molecular information, such as breakpoints and respective specific transcripts and introns/exons involved in the fusion event, along with the AGFusion plot. Each KGF annotation dataset also includes information on published experimental methods used for KGF validation with several supporting literature references and links to the Tumor Fusion Gene Data Portal database for corresponding KGF events detected in patient-derived tumor samples.

This section provides a graphical representation of the database content, in particular showing the distribution of kinase groups, tissues of origin for cell lines, tissues and types of aberrant chromosomal events that occurred to generate the KGFs.

The fusion data stored in the database can be exported as txt or sql files.
An application form in the upload page allows users to submit their experimentally validated kinase fusions not previously included in the database. Upon revision, data will be added to KuNG FU with the appropriate reference, if available, together with submitters’ acknowledgments.